Automated determination of red cell methaemoglobin reductase activity by a continuous-flow system for screening hereditary methaemoglobinaemia.
نویسندگان
چکیده
منابع مشابه
Automated determination of red cell methaemoglobin reductase activity by a continuous-flow system for screening hereditary methaemoglobinaemia.
A flow diagram for the automated determination of ferricyanide reductase activity in red blood cells was prepared in the modules from AutoAnalyzer AA I (Technicon Instruments Inc). Ferricyanide reductase assay can be substituted for assay of cytochrome b5 reductase (EC 1.6.2.2), which plays a major role in reducing methaemoglobin in erythrocytes, and is defective specifically in the erythrocyte...
متن کاملMethaemoglobin Content and NADH-Methaemoglobin Reductase Activity of Three Human Erythrocyte Genotypes
Background: To study methaemoglobin content and NADH-methaemoglobin reductase activity of three human erythrocyte genotypes (HbAA, HbAS and HbSS).Materials and Methods: Studies to ascertain methaemoglobin concentration and level of NADH-methaemoglobin reductase activity of three human erythrocyte genotypes (HbAA, HbAS and HbSS) were carried out in forty-three (43) healthy male participants of c...
متن کاملThe reduction of methaemoglobin in red blood cells and studies on the cause of idiopathic methaemoglobinaemia.
Biery, H. & Ranc, A. (1914). C.R. Acad. Sci., Pari8, 158, 278. Bywaters, H. W. (1909). Biochem. Z. 15, 322. Dumazert, C. & Levy, M. (1943). Bull. Soc. Chim. biol. 25, 1274. Dumazert, C., L6vy, M. & Marszak, I. (1944). Bull. Soc. Chim. biol. 26, 1214. Dumazert, C. & Memmi, A. (1943). Bull. Soc. chim. biol. 25, 1416. Falconer, J. S. & Taylor, D. B. (1946). Biochem. J. 40, 835. Gurin, S. & Hood, D...
متن کاملmethaemoglobin content and nadh-methaemoglobin reductase activity of three human erythrocyte genotypes
background: to study methaemoglobin content and nadh-methaemoglobin reductase activity of three human erythrocyte genotypes (hbaa, hbas and hbss).materials and methods: studies to ascertain methaemoglobin concentration and level of nadh-methaemoglobin reductase activity of three human erythrocyte genotypes (hbaa, hbas and hbss) were carried out in forty-three (43) healthy male participants of c...
متن کاملDual-channel continuous-flow system for determination of phenylalanine and galactose: application to newborn screening.
We describe a dual-channel AutoAnalyzer (Technicon) system for the simultaneous measurement of phenylalanine and galactose from blood specimens on filter-paper. Using a single 1/4-in.-diameter (6-mm) specimen, we measure both components fluorometrically at a rate of 70/h. Analytical recovery with the method and the linearity of measurements vs sample concentration are excellent through the rang...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1979
ISSN: 0021-9746
DOI: 10.1136/jcp.32.6.584